Multilocus phylogenetic analysis revealed a new cryptic lineage of <i>Serpula himantioides</i> in Japan

Serpula himantioides is a widely distributed saprotrophic fungus that causes root and butt rot in various tree species. In Japan, associated with S. observed Chamaecyparis pisifera Abies sachalinensis. Previous studies have shown includes five phylogenetically defined cryptic lineages, but the placement of Japanese isolates remains unclear. To clarify phylogenetic relationship between known we conducted analysis using three newly collected along 74 isolates, based on internal transcribed spacer (ITS) region, 28 S large subunit (LSU) rDNA, beta-tubulin (tub) gene, heat stress protein (hsp) gene. The concatenated showed composed clade high bootstrap value distinct from indicating present new lineage himantioides.